A Python based automated tracking routine for myosin II filaments
The study of motor protein dynamics within cytoskeletal networks is of high interest to physicists and biologists to understand how the dynamics and properties of individual motors lead to cooperative effects and control of overall network behaviour. Here, we report a method to detect and track muscular myosin II filaments within an actin network tethered to supported lipid bilayers. Based on the characteristic shape of myosin II filaments, this automated tracking routine allowed us to follow the position and orientation of myosin II filaments over time, and to reliably classify their dynamics into segments of diffusive and processive motion based on the analysis of displacements and angular changes between time steps. This automated, high throughput method will allow scientists to efficiently analyse motor dynamics in different conditions, and will grant access to more detailed information than provided by common tracking methods, without any need for time consuming manual tracking or generation of kymographs.
Code (0)
등록된 구현이 없습니다.
Similar Papers 제목 키워드 기반
Mechanosensitive Self-Assembly of Myosin II Minifilaments
Self-assembly and force generation are two central processes in biological systems that usually are considered in separation. However, the signals that activate non-muscle myosin II molecular motors simultaneously lead t…
A mechanism for sarcomere breathing: volume change and advective flow within the myofilament lattice
During muscle contraction, myosin motors anchored to thick filaments bind to and slide actin thin filaments. These motors rely on energy derived from ATP, supplied, in part, by diffusion from the sarcoplasm to the interi…
Filament turnover is essential for continuous long range contractile flow in a model actomyosin cortex
In this paper, we develop and analyze a minimal model for a 2D network of cross-linked actin filaments and myosin motors, representing the cortical cytoskeleton of eukaryotic cells. We implement coarse-grained representa…
FrictionActin reorganization throughout the cell cycle mediated by motor proteins
Cortical actin networks are highly dynamic and play critical roles in shaping the mechanical properties of cells. The actin cytoskeleton undergoes significant reorganization over the course of the cell cycle, when cortic…
Advecting Scaffolds: Controlling The Remodelling Of Actomyosin With Anillin
We propose and analyse an active hydrodynamic theory that characterises the effects of the scaffold protein anillin. Anillin is found at major sites of cortical activity, such as adherens junctions and the cytokinetic fu…