Correcting Illumina sequencing errors for human data
Summary: We present a new tool to correct sequencing errors in Illumina data produced from high-coverage whole-genome shotgun resequencing. It uses a non-greedy algorithm and shows comparable performance and higher accuracy in an evaluation on real human data. This evaluation has the most complete collection of high-performance error correctors so far. Availability and implementation: https://github.com/lh3/bfc Contact: hengli@broadinstitute.org
Code (1)
Similar Papers 제목 키워드 기반
Deep Neural Network: An Efficient and Optimized Machine Learning Paradigm for Reducing Genome Sequencing Error
Genomic data I used in many fields but, it has become known that most of the platforms used in the sequencing process produce significant errors. This means that the analysis and inferences generated from these data may …
BIG-bench Machine LearningCRANE: Correcting Errors in Raw Nanopore Signals Using Hidden Markov Models
Nanopore sequencing can read substantially longer sequences of nucleic acid molecules, called reads, than other sequencing methods, which has led to advances in genomic analysis such as the gapless human genome assembly.…
Microbiome profiling by Illumina sequencing of combinatorial sequence-tagged PCR products
We developed a low-cost, high-throughput microbiome profiling method that uses combinatorial sequence tags attached to PCR primers that amplify the rRNA V6 region. Amplified PCR products are sequenced using an Illumina p…
DiversityMeFiT: Merging and Filtering Tool for Illumina Paired-End Reads for 16S rRNA Amplicon Sequencing
Recent advances in next-generation sequencing have revolutionized genomic research. 16S rRNA amplicon sequencing using paired-end sequencing on the MiSeq platform from Illumina, Inc., is being used to characterize the co…
Extraction of long k-mers using spaced seeds
The extraction of k-mers from sequencing reads is an important task in many bioinformatics applications, such as all DNA sequence analysis methods based on de Bruijn graphs. These methods tend to be more accurate when th…