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New Sample Preparation Method for Transmission Electron Microscopy (TEM)

Cite this article: Deng, YH. Sublimable materials facilitate the TEM sample preparation of oil-soluble nanomaterials. Appl. Microsc. 50, 21 (2020). https://doi.org/10.1186/s42649-020-00042-7 Abstract: Sample preparation is significantly important to the high-resolution transmission electron microscopy (HRTEM) characterization of nanomaterials. However, many general organic solvents can dissolve the necessary organic polymer support layer in TEM grid, which causes it difficult to obtain high-quality samples of oil-soluble nanomaterials. In this study, a new sample preparation method for oil-soluble nanomaterials has been developed by using the sublimable material as a transition layer. Experiments also show that there is no damage to TEM grids and high-quality HRTEM images can be obtained via this method. This approach paves the way to applicable HRTEM sample preparation of oil-soluble nanomaterials. Results and discussion: Here, a new method for nanomaterial sample preparation was designed by introduce sublimable naphthalene as the transition layer. First, naphthalene was deposited on the substrate and nanomaterials were sprayed onto naphthalene layer. Next, the side of the naphthalene layer with the nanomaterial was transferred to covered on the TEM grid, and then place the sample in a vacuum to sublimate naphthalene. The process is shown in Fig. 2. Based on this method, we obtained a continuous large-scale highly-dispersed oil-soluble PbS nanocrystal sample and captured high-quality TEM (Fig. 3a), High-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM) (Fig. 3b) and HRTEM (Fig. 3c) images. It proves that images with low background contrast, accurate material morphology, and clear lattice fringes can be obtained via this new sample preparation method.

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