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Optical Diffraction Tomography Meets Fluorescence Localization Microscopy

2023-07-18 · Thanh-an Pham, Emmanuel Soubies, Ferréol Soulez, Michael Unser

We show that structural information can be extracted from single molecule localization microscopy (SMLM) data. More precisely, we reinterpret SMLM data as the measures of a phaseless optical diffraction tomography system for which the illumination sources are fluorophores within the sample. Building upon this model, we propose a joint optimization framework to estimate both the refractive index map and the position of fluorescent molecules from the sole SMLM frames.

📄 PDF Abstract BibTeX arXiv:2307.09261

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