Parallel G-duplex and C-duplex DNA with Uninterrupted Spines of AgI-Mediated Base Pairs
Hydrogen bonding between nucleobases produces diverse DNA structural motifs, including canonical duplexes, guanine (G) quadruplexes and cytosine (C) i-motifs. Incorporating metal-mediated base pairs into nucleic acid structures can introduce new functionalities and enhanced stabilities. Here we demonstrate, using mass spectrometry (MS), ion mobility spectrometry (IMS) and fluorescence resonance energy transfer (FRET), that parallel-stranded structures consisting of up to 20 G-Ag(I)-G contiguous base pairs are formed when natural DNA sequences are mixed with silver cations in aqueous solution. FRET indicates that duplexes formed by poly(cytosine) strands with 20 contiguous C-Ag(I)-C base pairs are also parallel. Silver-mediated G-duplexes form preferentially over G-quadruplexes, and the ability of Ag+ to convert G-quadruplexes into silver-paired duplexes may provide a new route to manipulating these biologically relevant structures. IMS indicates that G-duplexes are linear and more rigid than B-DNA. DFT calculations were used to propose structures compatible with the IMS experiments. Such inexpensive, defect-free and soluble DNA-based nanowires open new directions in the design of novel metal-mediated DNA nanotechnology.
Code (0)
등록된 구현이 없습니다.
Similar Papers 제목 키워드 기반
DuplexMamba: Enhancing Real-time Speech Conversations with Duplex and Streaming Capabilities
Real-time speech conversation is essential for natural and efficient human-machine interactions, requiring duplex and streaming capabilities. Traditional Transformer-based conversational chatbots operate in a turn-based …
Automatic Speech RecognitionAutomatic Speech Recognition (ASR)Language ModelingLanguage Modelling+4DuplexChat: Constructing Speaker-Separated Full-Duplex Dialogue Speech at Scale for Spoken Dialogue Language Modeling
Full-duplex spoken dialogue models are trained on conversational speech in which each speaker is represented as a separate stream, but existing large-scale public speech corpora are mostly monaural, making them unsuited …
Speech SeparationA Multi-Stage Duplex Fusion ConvNet for Aerial Scene Classification
Existing deep learning based methods effectively prompt the performance of aerial scene classification. However, due to the large amount of parameters and computational cost, it is rather difficult to apply these methods…
Aerial Scene ClassificationScene ClassificationDuplexCascade: Full-Duplex Speech-to-Speech Dialogue with VAD-Free Cascaded ASR-LLM-TTS Pipeline and Micro-Turn Optimization
Spoken dialog systems with cascaded ASR-LLM-TTS modules retain strong LLM intelligence, but VAD segmentation often forces half-duplex turns and brittle control. On the other hand, VAD-free end-to-end model support full-d…
A Research Journey of Full-Duplex at University of California from Self-Interference Cancellation to Wireless Network Security
This article provides an overview of research on full-duplex at the University of California, Riverside, in the past decade. This research was initially focused on self-interference (SI) cancellation, then moved to appli…