Quantitative effects of the stress response to DNA damage in the cell size control of Escherichia coli
In Escherichia coli the response to DNA damage shows strong cell-to-cell-heterogenity. This results in a random delay in cell division and asymmetrical binary fission of single cells, which can compromise the size homeostasis of the population. To quantify the effect of the heterogeneous response to genotoxic stress (called SOS response in E. coli) on the growth of the bacterial population, we propose a flexible time-continuous parametric model of individual-based population dynamics. We construct a stochastic model based on the "adder" size-control mechanism, extended to incorporate the dynamics of the SOS response and its effect on cell division. The model is fitted to individual lineage data obtained in a 'mother machine' microfluidic device. We show that the heterogeneity of the SOS response can bias the observed division rate. In particular, we show that the adder division rate is decreased by SOS induction and that this perturbative effect is stronger in fast-growing conditions.
Code (0)
등록된 구현이 없습니다.
Similar Papers 제목 키워드 기반
Yeast caspase 1 suppresses the burst of reactive oxygen species and maintains mitochondrial stability in Saccharomyces cerevisiae
Caspases are a family of cysteine proteases that play essential roles during apoptosis, and we presume some of them may also protect the cell from oxidative stress. We found that the absence of yeast caspase 1(Yca1)in Sa…
Endothelial Cell-specific Loss of Breast Cancer Susceptibility Gene 2 Exacerbates Atherosclerosis
The BReast CAncer type 2 susceptibility protein (BRCA2) responds to DNA damage by participating in homology-directed repair. BRCA2 deficiency culminates in defective DNA damage repair (DDR) that when prolonged leads to t…
Protein FoldingPDCD5 interacts with p53 and functions as a regulator of p53 dynamics in the DNA damage response
The tumor suppressor p53 plays a central role in cell fate decisions after DNA damage. Programmed Cell Death 5 (PDCD5) is known to interact with the p53 pathway to promote cell apoptosis. Recombinant human PDCD5 can sign…
The Transcription Factor E4F1 Coordinates CHK1-Dependent Checkpoint and Mitochondrial Functions
Recent data support the notion that a group of key transcriptional regulators involved in tumorigenesis, including MYC, p53, E2F1, and BMI1, share an intriguing capacity to simultaneously regulate metabolism and cell cyc…
Emerging classes of antioxidant to cancer therapy: a review of clinical and experimental studies
This review mainly focuses on the relation between antioxidants with cancer therapy. Antioxidants have been reported to play an essential role to reduce free radical species. Free radicals commonly cause oxidative damage…