Single NV in nanodiamond for quantum sensing of protein dynamics in an ABEL trap
Enzymes are cellular protein machines using a variety of conformational changes to power fast biochemical catalysis. Our goal is to exploit the single-spin properties of the luminescent NV (nitrogen-vacancy) center in nanodiamonds to reveal the dynamics of an active enzyme complex at physiological conditions with the highest spatio-temporal resolution. Specifically attached to the membrane enzyme FoF1-ATP synthase, the NV sensor will report the adenosine triphosphate (ATP)-driven full rotation of Fo motor subunits in ten consecutive 36{\deg} steps. Conformational dynamics are monitored using either a double electron-electron resonance scheme or NV- magnetometry with optical readout or using NV- relaxometry with a superparamagnetic nanoparticle as the second marker attached to the same enzyme. First, we show how all photophysical parameters like individual size, charge, brightness, spectral range of fluorescence and fluorescence lifetime can be determined for the NV- center in a single nanodiamond held in aqueous solution by a confocal anti-Brownian electrokinetic trap (ABEL trap). Stable photon count rates of individual nanodiamonds and the absence of blinking allow for observation times of single nanodiamonds in solution exceeding hundreds of seconds. For the proposed quantum sensing of nanometer-sized distance changes within an active enzyme, we show that local magnetic field fluctuations can be detected all-optically by analyzing fluorescence lifetime changes of the NV- center in each nanodiamond in solution.
Code (0)
등록된 구현이 없습니다.
Similar Papers 제목 키워드 기반
Machine-learning-enhanced quantum sensors for accurate magnetic field imaging
Local detection of magnetic fields is crucial for characterizing nano- and micro-materials and has been implemented using various scanning techniques or even diamond quantum sensors. Diamond nanoparticles (nanodiamonds) …
BIG-bench Machine LearningTowards ubiquitous radio access using nanodiamond based quantum receivers
The development of sixth-generation (6G) wireless communication systems demands innovative solutions to address challenges in the deployment of a large number of base stations and the detection of multi-band signals. Qua…
Machine learning frontier orbital energies of nanodiamonds
Nanodiamonds have a wide range of applications including catalysis, sensing, tribology and biomedicine. To leverage nanodiamond design via machine learning, we introduce the new dataset ND5k, consisting of 5,089 diamondo…
Graph Neural NetworkNanodiamond quantum sensors reveal temperature variation associated to hippocampal neurons firing
Temperature is one of the most relevant parameters for the regulation of intracellular processes. Measuring localized subcellular temperature gradients is fundamental for a deeper understanding of cell function, such as …
Nanopores -- a Versatile Tool to Study Protein Dynamics
Proteins are the active working horses in our body. These biomolecules perform all vital cellular functions from DNA replication and general biosynthesis to metabolic signaling and environmental sensing. While static 3D …