The cellular uptake mechanism of SPIONs: an in-vitro study
The Superparamagnetic Iron Oxide Nanoparticles (SPIONs) of sizes ranging from 10-50 nm are being used in a large number of biological studies because of their peculiar characteristics of inducing local hyperthermia, MR imaging, specific targeting and drug delivery. An in-vitro study of the cytotoxicity and an understanding of the specific pathway of cellular uptake will enable manipulation of conditions for optimal cellular uptake of SPIONs for targeted therapy. The objective of the present study was to identify the endocytotic pathway through which the SPIONs are taken up by C6 glioma cells. The cells were pre-incubated with different concentrations of pharmacological inhibitors and then exposed to SPIONs for a few hours. The endocytosed particles were localized and quantitatively estimated using Perl's or Prussian Blue reaction. There was significant reduction in the uptake of SPIONs when incubated with the inhibitor indicating the uptake of nanoparticles is being inhibited. This reduction in SPION uptake was found to be dependent on the concentration of the inhibitors and also the nature of the inhibitors. By a systematic study of choosing various inhibitors, the data can be narrowed down to the final pathway that may be involved in the SPION uptake. The present preliminary investigation is expected to provide insight of testing the possible mechanisms with complementary techniques.
Code (0)
등록된 구현이 없습니다.
Similar Papers 제목 키워드 기반
Hybrid Machine Learning and Mathematical Modeling for Tumor Dynamics Prediction: Comparing SPIONs against mNP-FDG
This is a Machine Learning guided study towards zone-specific ray therapy. Combining Machine Learning (Extreme Gradient Boosting) with continuum modeling (exponential and logistic growth), we find that while fluorodeoxyg…
Hybrid Machine LearningVirtual Electrode Recording Tool for EXtracellular potentials (VERTEX): Comparing multi-electrode recordings from simulated and biological mammalian cortical tissue
Local field potentials (LFPs) sampled with extracellular electrodes are frequently used as a measure of population neuronal activity. However, relating such measurements to underlying neuronal behaviour and connectivity …
Graphene oxide upregulates the homeostatic functions of primary astrocytes and modulates astrocyte-to-neuron communication
Graphene-based materials are the focus of intense research efforts to devise novel theranostic strategies for targeting the central nervous system. In this work, we have investigated the consequences of long-term exposur…
Effects of mesoporous SiO2-CaO nanospheres on the murine peritoneal macrophages -- Candida albicans interface
The use of nanoparticles for intracellular drug delivery could reduce the toxicity and side effects of the drug but, the uptake of these nanocarriers could induce adverse effects on cells and tissues after their incorpor…
In Vitro Vascularized Tumor Platform for Modeling Tumor-Vasculature Interactions of Inflammatory Breast Cancer
Inflammatory breast cancer (IBC), a rare form of breast cancer associated with increased angiogenesis and metastasis, is largely driven by tumor-stromal interactions with the vasculature and the extracellular matrix (ECM…