Designing and simulating realistic spatial frequency domain imaging systems using open-source 3D rendering software
Spatial frequency domain imaging (SFDI) is a low-cost imaging technique that can deliver real-time maps of absorption and reduced scattering coefficients. However, there are a wide range of imaging geometries that practical SFDI systems must cope with including imaging flat samples ex vivo, imaging inside tubular lumen in vivo such as in an endoscopy, and measuring tumours or polyps of varying shapes, sizes and optical properties. There is a need for a design and simulation tool to accelerate design and fabrication of new SFDI systems. We present such a system implemented using open-source 3D design and ray-tracing software Blender that is capable of simulating media with realistic optical properties (mimicking healthy and cancerous tissue), a wide variety of shapes and size, and in both planar and tubular imaging geometries. We first demonstrate quantitative agreement between Monte-Carlo simulated scattering and absorption coefficients and those measured from our Blender system. Next, we show the ability of the system to simulate absorption, scattering and shape for flat samples with small simulated tumours and show that the improved contrast associated with SFDI is reproduced. Finally, to demonstrate the versatility of the system as a design tool we show that it can be used to generate a custom look-up-table for mapping from modulation amplitude values to absorption and scattering values in a tubular geometry, simulating a lumen. As a demonstrative example we show that longitudinal sectioning of the tube, with separate look-up tables for each section, significantly improves accuracy of SFDI, representing an important design insight for future systems. We therefore anticipate our simulation system will significantly aid in the design and development of novel SFDI systems, especially as such systems are miniaturised for deployment in endoscopic and laparoscopic systems.
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