Development of Pipetting Devices to Separate Protein Complexes
The objective of this project is to develop an automated device used to spot protein samples on a hydrophobic membrane to be used for the patented electrophoresis method developed by Chang and Yonan in 2008 [1]. This novel method performs electrophoresis directly on hydrophobic blot membranes as opposed to the previous popular methods such as the 2-D polyacrylamide gel method [2, 3]. This new electrophoresis method significantly reduces the processing time to about 40 minutes, as opposed to the 2-D PAGE method which can take one or two days. Special methods to spot samples on hydrophobic blot membranes were established for successful separation of protein and protein complexes. These spotting methods are used in conjunction with the patented electrophoresis method [1] for effective separation. The automated device effectively replicates these special methods that are repeatable and user independent. The goal of the automated device is to effectively spot and separate protein complexes in addition to single proteins under non-denaturing conditions, thus eliminating the variability of the manual spotting method.
Code (0)
등록된 구현이 없습니다.
Similar Papers 제목 키워드 기반
iTreePack: Protein Complex Side-Chain Packing by Dual Decomposition
Protein side-chain packing is a critical component in obtaining the 3D coordinates of a structure and drug discovery. Single-domain protein side-chain packing has been thoroughly studied. A major challenge in generalizin…
Drug DiscoveryTree DecompositionInformation-Driven Modeling of Biomolecular Complexes
Proteins play crucial roles in every cellular process by interacting with each other, with nucleic acids, metabolites, and other molecules. The resulting assemblies can be very large and intricate and pose challenges to …
Molecular DockingAn All-Atom Generative Model for Designing Protein Complexes
Proteins typically exist in complexes, interacting with other proteins or biomolecules to perform their specific biological roles. Research on single-chain protein modeling has been extensively and deeply explored, with …
AllEGR: Equivariant Graph Refinement and Assessment of 3D Protein Complex Structures
Protein complexes are macromolecules essential to the functioning and well-being of all living organisms. As the structure of a protein complex, in particular its region of interaction between multiple protein subunits (…
Drug DiscoveryGraph Neural Network3D Reconstruction of Protein Complex Structures Using Synthesized Multi-View AFM Images
Recent developments in deep learning-based methods demonstrated its potential to predict the 3D protein structures using inputs such as protein sequences, Cryo-Electron microscopy (Cryo-EM) images of proteins, etc. Howev…
3D Reconstruction8k