Direct quantification of brown algae-derived fucoidans in human plasma by a fluorescent probe assay
Fucoidan is a generic term for a class of fucose rich, structurally diverse sulfated polysaccharides that are found in brown algae and other marine organisms. Depending on the species from which the fucoidan is extracted, a wide variety of biological activities including antitumor, antiinflammatory, immune-modulating, antiviral, antibacterial and pro- and anticoagulant activities has been described. Fucoidans have the advantage of low toxicity and oral bioavailibiity and are viable drug candidates, preclinical and pilot clinical trials show promising results. The availability of robust assays, in particular for analysing the blood levels of fucoidan, is a fundamental requirement for pharmacokinetic analysis in drug development projects. This contribution describes the application of a commercially availbale, protein-free fluorescent probe assay (Heparin Red) for the direct quantification of several fucoidans (from Fucus vesiculosus, Macrocystis pyrifera, and Undaria pinnatifida) in human plasma. By only minor adapation of the established protocol for heparin detection, a concentration range 0,5 to 20 microgram per mL fucoidan can be addressed. A preliminary analysis of matrix effects suggests acceptable interindividual variability and no interference by endogeneous chondroitin sulfate. This study identifies the Heparin Red assay as a simple, time-saving mix-and-read method for the quantification of fucoidans in human plasma.
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